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KMID : 0903520070500040327
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2007 Volume.50 No. 4 p.327 ~ p.333
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Antioxidant Activities of Extract with Water and Ethanol of Perilla frutescens var. acuta kudo Leaf
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Kim Mi-Hyang
Kang Woo-Won
Lee Nan-Hee
Kwoen Dae-Jun
Choi Ung-Kyu
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Abstract
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This study was conducted to examine antioxidant activities of Perilla frutescens var. acuta leaf. For the this purpose, DPPH radical scavenging activity, lipid oxidation inhibition, SOD-like activity, and xanthine oxidase inhibitor activity of water extract, ethanol extracts (30, 50, 70, and 95%) and the fractions obtained from these extracts were determined. The electron donating abilities of the chlo- roform fraction obtained from the 70% and 95% ethanol extracts were 50%, and that of the ethyl acetate fraction for all of the extracts was above 75%. In particular, the electron donating ability of the ethyl acetate fraction of the 70% ethanol extract showed the greatest activity with 200.5 ppm of RC50 value. The 70% ethanol extract was most effective to inhibit the automatic oxidation of linoleic acid at 40o C storage. The highest inhibition effects appeared in the chloroform and ethyl acetate fractions of the water extract, and the 30, 50, and 70% ethanol extracts, and the highest lipid oxidation inhibiting effect of the 95% ethanol extract occurred in the hexane and acetate frac- tions. The SOD-like activity of the water extract was 30.3%, and the activities of the various con- centration of ethanol extracts were 28-32% and the activity of the 70% ethanol extract was the highest. The SOD-like activity of the ethyl acetate fraction of the 70% ethanol extract was highest with 1,549.0 ppm of IC50 value. Xanthine oxidase inhibition activity was greatest in the water extract and the activities of the ethanol extracts were 36-41.2%. The xanthine oxidase inhibition activity of the ethyl acetate fraction of the water extract was highest. In summary, we found that electron donating ability, lipid oxidation inhibition, and SOD-like activity of Perilla frutescens var. acuta leaf were greatest in the ethyl acetate fraction of the 70% ethanol extract, and xanthine oxi- dase inhibition activity was highest in the ethyl acetate fraction of the water extract.
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KEYWORD
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Perilla frutescens var, acuta kudo, electron donating activity, SOD-like activity, xanthine oxidase
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